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KMID : 0355519920160010036
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Korean Journal of Oral Biology
1992 Volume.16 No. 1 p.36 ~ p.44
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Effect of excitatory amino acid on the neuronal activity of trigeminal spinal sensory nucleus in vitro
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Yoon Chan-Song
Kim Joong-Soo
Lee Song-Heun
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Abstract
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This study was performed to elucidate the effects of excitatory amino acid(EAA) on medullary dorsal horn neurons in vitro and the interelations between EAA and substance P in medullary dorsal horn. Horizontal slices of caudal part of trigeminal
sensory nucleus(500-600㎛thick) were prepared from rats about 5 weeks old, then transferred and fixed in the recording chamber where it was preincubated and continuously perfused with aerated modified Krebs-Ringer solution. Intracellular recordings were carried out with glass microelectrode to record the changes of membrane potential and membrane input resistance induced by glutamate, aspartate and substance P perfusion. Glutamate and aspartate respectively depolarized medullary dorsal horn neurons and decreased membrane input resistance in a dose-dependent manner. Effect of glutamate and aspartate applied simultaneously on medullary dorsal horn neuron was not different from that of glutamate or aspartate, respectively. Substance P also depolarized medullary dorsal horn neurons and decreased membrane input resistance. Medullary dorsal horn neurons responding to glutamate also responded to aspartate and substance P.
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KEYWORD
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trigeminal spinal sensory nucleus, Medullary dorsal horn Glutamate, Aspartate, Substance P, Intracellular membrane potential, membrane input resistance
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